Description of feature
Long reads from ONT or PacBio are typically stored as unaligned BAM (uBAM) files, so it would be beneficial to enable uBAM input. Some tools already accept BAM input e.g. sequali, FastQC and ToulligQQ, but for others a uBAM to FASTQ conversion would be required.
uBAM often come with additional metadata that could be valuable for QC. This includes both run information e.g. read start time for ONT and methylation information (MM/ML tag) for each read which is missing from the FASTQs that would enable read methylation QC, for example using modkit.
Description of feature
Long reads from ONT or PacBio are typically stored as unaligned BAM (uBAM) files, so it would be beneficial to enable uBAM input. Some tools already accept BAM input e.g. sequali, FastQC and ToulligQQ, but for others a uBAM to FASTQ conversion would be required.
uBAM often come with additional metadata that could be valuable for QC. This includes both run information e.g. read start time for ONT and methylation information (MM/ML tag) for each read which is missing from the FASTQs that would enable read methylation QC, for example using modkit.