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Python package with functions to analyse data from Lab Pod module

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LabPod

LabPod is a small Python toolkit for lab data analysis - inspired by lab work on Imperial's Medical Biosciences BSc.

Currently includes protein_quantifier() for BSA standard curve fitting + protein concentration calculation from your fixed Excel layout - example below.

PyPI version Downloads

Collaboration

If you have any ideas for additional functions for the package or want to contribute to the package with your own code please email me - mh1623@ic.ac.uk

Install

pip install LabPod

How to use

In python file run:

from labpod import protein_quantifier
protein_quantifier("path to your excel file", number of protein samples)

Function works for 96-well plates loaded in the manner. alt text

Output

The protein_quantifier() function generates a BSA standard curve using linear regression and calculates the concentration of unknown protein samples from their absorbance values.

Example Results

  1. Once ran the graph will pop up and you are able to save.
  2. When you close the graph the protein concetrations of your samples will show in the terminal

The function returns:

  • The fitted regression equation
  • The coefficient of determination (R²)
  • A plotted BSA curve
  • The calculated protein concentration for each unknown sample (labeled as in the image above) - assuming you sample is the same econcetration as your stock.

About

Python package with functions to analyse data from Lab Pod module

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